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Member Research and Reports

South Carolina Researchers Use Nanoparticles to Learn When Toxicity Occurs in the Food Chain

The scientists at the University of South Carolina’s SmartState Center for Environmental Nanoscience and Risk (CENR) in the Arnold School of Public Health have one overarching goal: to harness the benefits of nanoparticles while minimizing possible side effects. Researchers at CENR, which is located within the Department of Environmental Health Sciences, are always working on multiple research angles to benefit environmental and human health through the strategic use of nanoparticles. For example, they have developed a revolutionary method for cleaning up oil pollution in water and soil using nanoparticles that reduces oil toxicity by almost 100 percent and is far simpler and much less expensive (80-90 percent reduction in costs) compared to current oil remediation methods.

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Another project involves using nanoparticles to understand the fundamental mechanisms of uptake into cells or organisms and toxicity under various conditions. They are working to learn more about how a nanoparticle enters a cell directly as a particle and under what conditions it breaks down (or dissolves, much like salt does in water) and the dissolved elements (ions) enter the cells of a plant or animal.

Using silver nanoparticles as an example, the team developed isotopically* labeled three-layer nanohybrids with a silver core (Ag107), an insoluble gold layer around the core, and then a soluble outer layer of silver (Ag109). The chemistry and biology of these isotopes are identical but they can be distinguished using a specialized piece of equipment called a single particle inductively coupled plasma mass spectrometer (SP-ICP-MS).  The outer layer of silver nanoparticles partly dissolves into the surrounding environment while the insoluble gold prevents dissolution of the silver core. The researchers then measure the Ag107 to Ag109 ratio to separate the direct and indirect uptake mechanisms. This approach allows them to assess the different paths to uptake of the silver into the food chain to learn more about how and why certain types of particles enter the food chain (e.g., wateràalgaeàoystersàmammals/ humans) and at what concentrations.

Employing a variety of state-of-the-art methods, the team is able to determine the amount and nature of silver present in a particular stage of the food chain by analyzing the composition of the nanohybrid particles (i.e., Has it dissolved? How much?). The SP-ICP-MS is manufactured by PerkinElmer®, who has been a critical research partner in developing new methods for the instrument. CENR has published their success with this approach in Talanta and NanoImpact. They have also published several application notes with PerkinElmer and have a patent pending in this area.

“The detailed work has taken several years to perfect and is at a stage now where we can use the nanoparticles as probes in real systems” say Dr. Ruth Merrifield, CENR Research Assistant Professor and first author on the paper. “We are now applying these methods to investigate how nanoaprticles might enter the human food chain and affect human health and environmental health”

Read more: http://www.sc.edu/study/colleges_schools/public_health/about/news/2016/nanoparticles_toxicity.php